High-pressure CO2 has been known to increase membrane permeability by introducing pores in the cell wall, therefore destroying the integrity of the cellular structure (Garcia-Gonzalez et al., 2010; Hong and Pyun., 2001) The damaging effect of pressurized CO2 on cell membranes has been demonstrated as having a direct relationship with inactivation of E.coli (Yao etal., 2014).Progressively longer exposure to CO2 has been linked with morphological changes (Wu et al, 2016). Biophysical effects of supercrticial CO2 will lead to a significant perturbation of membrane architecture in E.coli because it can dramatically decrease the phosphatidyglycerol (PG) membrane lipid which plays an important role in membrane stability (Tamburini etal., 2014).